journal of medical council of islamic republic of iran
مجله علمی پژوهشی سازمان نظام پزشکی جمهوری اسلامی ایران
jmciri
Medical Sciences
http://jmciri.ir
1
admin
1562-1073
0
8
7
14
8888
13
fa
jalali
1398
7
1
gregorian
2019
10
1
37
2
online
1
fulltext
fa
اثر هشت هفته تمرین هوازی و مقاومتی بر بیان ژن پروتئین کیناز فعال شده توسط (AMPK) AMP در عضله نعلی و مقاومت به انسولین موشهای صحرایی دیابتی شده توسط استرپتوزوتوسین
The Effect of Eight Weeks Aerobic and Resistance Training
on AMP-Activated Protein Kinase (AMPK) Gene
Expression in Soleus Muscle and Insulin Resistance of STZ-Induced Diabetic Rat
عمومى
General
پژوهشي
Research
<strong><span style="font-size:10.0pt;">زمینه</span></strong><strong><span dir="LTR"><span style="font-size:10.0pt;">:</span></span></strong> <span style="font-size:10.0pt;">تنظیم</span> <span style="font-size:10.0pt;">ژن</span><span dir="LTR"><span style="font-size:10.0pt;"> AMPK </span></span><span style="font-size:10.0pt;">یکی</span> <span style="font-size:10.0pt;">از</span> <span style="font-size:10.0pt;">بزرگترین</span> <span style="font-size:10.0pt;">اهداف</span> <span style="font-size:10.0pt;">در</span> <span style="font-size:10.0pt;">مطالعات</span><span dir="LTR"><span style="font-size:10.0pt;"> T2D </span></span><span style="font-size:10.0pt;">و</span> <span style="font-size:10.0pt;">سندروم</span> <span style="font-size:10.0pt;">متابولیک</span> <span style="font-size:10.0pt;">می</span><span dir="LTR"><span style="font-family:calibri,sans-serif;"><span style="font-size:10.0pt;"></span></span></span><span style="font-size:10.0pt;">باشد</span><span dir="LTR"><span style="font-size:10.0pt;">. </span></span><span style="font-size:10.0pt;">بنابراین</span> <span style="font-size:10.0pt;">هدف</span> <span style="font-size:10.0pt;">از</span> <span style="font-size:10.0pt;">تحقیق</span> <span style="font-size:10.0pt;">حاضر</span> <span style="font-size:10.0pt;">بررسی</span> <span style="font-size:10.0pt;">اثر</span> <span style="font-size:10.0pt;">هشت</span> <span style="font-size:10.0pt;">هفته</span> <span style="font-size:10.0pt;">تمرین</span> <span style="font-size:10.0pt;">هوازی</span> <span style="font-size:10.0pt;">و</span> <span style="font-size:10.0pt;">مقاومتی</span> <span style="font-size:10.0pt;">بر</span> <span style="font-size:10.0pt;">بیان</span> <span style="font-size:10.0pt;">ژن</span> <span style="font-size:10.0pt;">پروتئین</span> <span style="font-size:10.0pt;">کیناز</span> <span style="font-size:10.0pt;">فعال</span> <span style="font-size:10.0pt;">شده</span> <span style="font-size:10.0pt;">توسط</span><span dir="LTR"><span style="font-size:10.0pt;"> AMPK) AMP) </span></span><span style="font-size:10.0pt;">در</span> <span style="font-size:10.0pt;">عضله</span> <span style="font-size:10.0pt;">نعلی</span> <span style="font-size:10.0pt;">و</span> <span style="font-size:10.0pt;">مقاومت</span> <span style="font-size:10.0pt;">به</span> <span style="font-size:10.0pt;">انسولین</span> <span style="font-size:10.0pt;">موش</span><span dir="LTR"><span style="font-family:calibri,sans-serif;"><span style="font-size:10.0pt;"></span></span></span><span style="font-size:10.0pt;">های</span> <span style="font-size:10.0pt;">دیابتی</span> <span style="font-size:10.0pt;">شده</span> <span style="font-size:10.0pt;">توسط</span> <span style="font-size:10.0pt;">استرپتوزوتوسین</span> <span dir="LTR"><span style="font-family:minion pro,serif;"><span style="font-size:10.0pt;">Streptozotocin</span></span></span><span dir="LTR"><span style="font-size:10.0pt;"> (STZ) </span></span><span style="font-size:10.0pt;">بود</span><span dir="LTR"><span style="font-size:10.0pt;">.</span></span><br>
<strong><span style="font-size:10.0pt;">روش کار: </span></strong><span style="font-size:10.0pt;">تحقیق حاضر از نوع تجربی بود. </span><span dir="LTR"><span style="font-size:10.0pt;">21 </span></span><span style="font-size:10.0pt;">سر</span> <span style="font-size:10.0pt;">رت</span> <span style="font-size:10.0pt;">صحرایی</span> <span style="font-size:10.0pt;">نر</span> <span style="font-size:10.0pt;">نژاد</span> <span style="font-size:10.0pt;">ویستار</span> <span style="font-size:10.0pt;">با</span> <span style="font-size:10.0pt;">دامنه</span> <span style="font-size:10.0pt;">وزنی</span><span dir="LTR"><span style="font-size:10.0pt;"> 200 </span></span><span style="font-size:10.0pt;">تا</span><span dir="LTR"><span style="font-size:10.0pt;"> 250 </span></span><span style="font-size:10.0pt;">گرم</span> <span style="font-size:10.0pt;">و</span> <span style="font-size:10.0pt;">سن</span><span dir="LTR"><span style="font-size:10.0pt;"> 8 </span></span><span style="font-size:10.0pt;">تا</span><span dir="LTR"><span style="font-size:10.0pt;"> 10 </span></span><span style="font-size:10.0pt;">هفته</span> <span style="font-size:10.0pt;">به</span> <span style="font-size:10.0pt;">عنوان</span> <span style="font-size:10.0pt;">نمونه</span> <span style="font-size:10.0pt;">انتخاب</span> <span style="font-size:10.0pt;">شدند</span> <span style="font-size:10.0pt;">و</span> <span style="font-size:10.0pt;">با</span> <span style="font-size:10.0pt;">تزریق</span> <span style="font-size:10.0pt;">درون</span> <span style="font-size:10.0pt;">صفاقی</span> <span style="font-size:10.0pt;">محلول تازه تهیه شده </span><span dir="LTR"><span style="font-size:10.0pt;">STZ</span></span><span style="font-size:10.0pt;"> دیابتی شده و به طور تصادفی در سه گروه دیابت کنترل، دیابت تمرین مقاومتی و دیابت تمرین هوازی قرار داده شدند. برنامه تمرینی گروه</span><span dir="LTR"><span style="font-family:calibri,sans-serif;"><span style="font-size:10.0pt;"></span></span></span><span style="font-size:10.0pt;">های تمرینی برای 8 هفته و 5 روز در هفته انجام شد. پروتکل تمرین مقاومتی در قالب 10 تکرار به صورت صعود از نردبان با وزنه</span><span dir="LTR"><span style="font-family:calibri,sans-serif;"><span style="font-size:10.0pt;"></span></span></span><span style="font-size:10.0pt;">ای به مقدار 100 درصد وزن بدن و پروتکل تمرین هوازی دویدن روی نوارگردان با سرعت 25 متر در دقیقه به مدت 40 دقیقه بود. 48 ساعت پس از آخرین جلسه تمرین، عضله نعلی رتها جدا و میزان بیان ژن </span><span dir="LTR"><span style="font-size:10.0pt;">AMPK</span></span><span style="font-size:10.0pt;"> نمونه</span><span dir="LTR"><span style="font-family:calibri,sans-serif;"><span style="font-size:10.0pt;"></span></span></span><span style="font-size:10.0pt;">ها سنجیده شد. تجزیه و تحلیل داده</span><span dir="LTR"><span style="font-family:calibri,sans-serif;"><span style="font-size:10.0pt;"></span></span></span><span style="font-size:10.0pt;">های آماری از</span><span style="font-size:10.0pt;">طریق</span> <span style="font-size:10.0pt;">آنالیز</span> <span style="font-size:10.0pt;">واریانس</span> <span style="font-size:10.0pt;">یک</span> <span style="font-size:10.0pt;">طرفه</span><span dir="LTR"><span style="font-size:10.0pt;"> (One Way Anova)</span></span><span style="font-size:10.0pt;"> در سطح معناداری 05/0></span><span dir="LTR"><span style="font-size:10.0pt;">P</span></span><span style="font-size:10.0pt;"> انجام شد</span><span dir="LTR"><span style="font-size:10.0pt;">. </span></span><br>
<strong><span style="font-size:10.0pt;">یافته</span></strong><strong><span dir="LTR"><span style="font-family:calibri,sans-serif;"><span style="font-size:10.0pt;"></span></span></span></strong><strong><span style="font-size:10.0pt;">ها</span></strong><strong><span dir="LTR"><span style="font-size:10.0pt;">:</span></span></strong> <span style="font-size:10.0pt;">نتایج تحقیق حاضر نشان دادکه میزان بیان ژن پروتئین </span><span dir="LTR"><span style="font-size:10.0pt;">AMPK </span></span><span style="font-size:10.0pt;">در</span> <span style="font-size:10.0pt;">عضله</span> <span style="font-size:10.0pt;">نعلی</span> <span style="font-size:10.0pt;">گروه</span> <span style="font-size:10.0pt;">تمرین</span> <span style="font-size:10.0pt;">هوازی</span> <span style="font-size:10.0pt;">و</span> <span style="font-size:10.0pt;">تمرین</span> <span style="font-size:10.0pt;">مقاومتی</span> <span style="font-size:10.0pt;">به</span> <span style="font-size:10.0pt;">طور</span> <span style="font-size:10.0pt;">معناداری</span> <span style="font-size:10.0pt;">بالاتر</span> <span style="font-size:10.0pt;">از</span> <span style="font-size:10.0pt;">گروه</span> <span style="font-size:10.0pt;">کنترل</span> <span style="font-size:10.0pt;">بود</span><span dir="LTR"><span style="font-size:10.0pt;"> (</span></span><span style="font-size:10.0pt;">مقادیر</span><span dir="LTR"><span style="font-size:10.0pt;"> P </span></span><span style="font-size:10.0pt;">به</span> <span style="font-size:10.0pt;">ترتیب</span><span dir="LTR"><span style="font-size:10.0pt;"> 0/035 </span></span><span style="font-size:10.0pt;">و</span><span dir="LTR"><span style="font-size:10.0pt;"> 0/019). </span></span><span style="font-size:10.0pt;">اما</span> <span style="font-size:10.0pt;">بین</span> <span style="font-size:10.0pt;">سطح</span> <span style="font-size:10.0pt;">این</span> <span style="font-size:10.0pt;">شاخص</span> <span style="font-size:10.0pt;">در</span> <span style="font-size:10.0pt;">گروه</span><span dir="LTR"><span style="font-family:calibri,sans-serif;"><span style="font-size:10.0pt;"></span></span></span><span style="font-size:10.0pt;">های</span> <span style="font-size:10.0pt;">تمرین</span> <span style="font-size:10.0pt;">هوازی</span> <span style="font-size:10.0pt;">و</span> <span style="font-size:10.0pt;">مقاومتی</span> <span style="font-size:10.0pt;">تفاوت</span> <span style="font-size:10.0pt;">معناداری</span> <span style="font-size:10.0pt;">مشاهده</span> <span style="font-size:10.0pt;">نشد</span><span dir="LTR"><span style="font-size:10.0pt;"> (0/954=P). </span></span><span style="font-size:10.0pt;">سطح گلوکز خون ناشتا در گروه</span><span dir="LTR"><span style="font-family:calibri,sans-serif;"><span style="font-size:10.0pt;"></span></span></span><span style="font-size:10.0pt;">های دیابت تمرین هوازی و دیابت تمرین مقاومتی نسبت به گروه دیابت کنترل به طور معناداری پایین</span><span dir="LTR"><span style="font-family:calibri,sans-serif;"><span style="font-size:10.0pt;"></span></span></span><span style="font-size:10.0pt;">تر بود (</span><span dir="LTR"><span style="font-size:10.0pt;">0/001=P). </span></span><span style="font-size:10.0pt;">همچنین</span> <span style="font-size:10.0pt;">سطح</span> <span style="font-size:10.0pt;">انسولین و مقاومت به انسولین در گروه دیابت تمرین هوازی و گروه دیابت تمرین مقاومتی نسبت به گروه کنترل به طور معناداری پایین</span><span dir="LTR"><span style="font-family:calibri,sans-serif;"><span style="font-size:10.0pt;"></span></span></span><span style="font-size:10.0pt;">تر بود (05</span><span dir="LTR"><span style="font-size:10.0pt;">/0>P</span></span><span style="font-size:10.0pt;">). </span><br>
<strong><span style="font-size:10.0pt;">نتیجه</span></strong><strong><span dir="LTR"><span style="font-family:calibri,sans-serif;"><span style="font-size:10.0pt;"></span></span></span></strong><strong><span style="font-size:10.0pt;">گیری</span></strong>: <span style="font-size:10.0pt;">به</span> <span style="font-size:10.0pt;">طور</span> <span style="font-size:10.0pt;">کلی،</span> <span style="font-size:10.0pt;">نتایج</span> <span style="font-size:10.0pt;">این</span> <span style="font-size:10.0pt;">تحقیق</span> <span style="font-size:10.0pt;">نشان</span> <span style="font-size:10.0pt;">می</span><span dir="LTR"><span style="font-family:calibri,sans-serif;"><span style="font-size:10.0pt;"></span></span></span><span style="font-size:10.0pt;">دهد</span> <span style="font-size:10.0pt;">که</span> <span style="font-size:10.0pt;">هم</span> <span style="font-size:10.0pt;">تمرین</span> <span style="font-size:10.0pt;">هوازی</span> <span style="font-size:10.0pt;">و</span> <span style="font-size:10.0pt;">هم</span> <span style="font-size:10.0pt;">تمرین</span> <span style="font-size:10.0pt;">مقاومتی</span> <span style="font-size:10.0pt;">از</span> <span style="font-size:10.0pt;">طریق</span> <span style="font-size:10.0pt;">افزایش</span> <span style="font-size:10.0pt;">سطح</span><span dir="LTR"><span style="font-size:10.0pt;"> AMPK</span></span><span style="font-size:10.0pt;">،</span> <span style="font-size:10.0pt;">موجب</span> <span style="font-size:10.0pt;">کاهش</span> <span style="font-size:10.0pt;">مقاومت</span> <span style="font-size:10.0pt;">به</span> <span style="font-size:10.0pt;">انسولین</span> <span style="font-size:10.0pt;">و</span> <span style="font-size:10.0pt;">در</span> <span style="font-size:10.0pt;">نتیجه</span> <span style="font-size:10.0pt;">بهبود</span> <span style="font-size:10.0pt;">سطح</span> <span style="font-size:10.0pt;">گلوکز</span> <span style="font-size:10.0pt;">خون</span> <span style="font-size:10.0pt;">در</span> <span style="font-size:10.0pt;">موش</span><span dir="LTR"><span style="font-family:calibri,sans-serif;"><span style="font-size:10.0pt;"></span></span></span><span style="font-size:10.0pt;">های</span> <span style="font-size:10.0pt;">صحرایی</span> <span style="font-size:10.0pt;">دیابتی</span> <span style="font-size:10.0pt;">می</span><span dir="LTR"><span style="font-family:calibri,sans-serif;"><span style="font-size:10.0pt;"></span></span></span><span style="font-size:10.0pt;">گردند</span><span dir="LTR"><span style="font-size:10.0pt;">. </span></span><span style="font-size:10.0pt;">بر</span> <span style="font-size:10.0pt;">این</span> <span style="font-size:10.0pt;">اساس</span> <span style="font-size:10.0pt;">میتوان</span> <span style="font-size:10.0pt;">گفت</span> <span style="font-size:10.0pt;">که</span> <span style="font-size:10.0pt;">تمرین</span> <span style="font-size:10.0pt;">هوازی</span> <span style="font-size:10.0pt;">و</span> <span style="font-size:10.0pt;">مقاومتی</span> <span style="font-size:10.0pt;">باعث</span> <span style="font-size:10.0pt;">فعال</span> <span style="font-size:10.0pt;">سازی</span> <span style="font-size:10.0pt;">مسیر</span> <span style="font-size:10.0pt;">غیر</span> <span style="font-size:10.0pt;">وابسته</span> <span style="font-size:10.0pt;">به</span> <span style="font-size:10.0pt;">انسولین</span> <span style="font-size:10.0pt;">شده</span> <span style="font-size:10.0pt;">و</span> <span style="font-size:10.0pt;">بهبودی</span> <span style="font-size:10.0pt;">در</span> <span style="font-size:10.0pt;">مقاومت</span> <span style="font-size:10.0pt;">به</span> <span style="font-size:10.0pt;">انسولین</span> <span style="font-size:10.0pt;">در</span> <span style="font-size:10.0pt;">رتهای</span> <span style="font-size:10.0pt;">دیابتی</span> <span style="font-size:10.0pt;">را</span> <span style="font-size:10.0pt;">به</span> <span style="font-size:10.0pt;">همراه</span> <span style="font-size:10.0pt;">دارد</span><span dir="LTR"><span style="font-size:10.0pt;">.</span></span>
Background: AMPK regulation is one of biggest target in T2D and metabolic syndrome research. Therefore, the present study is aimed to investigate The effect of 8 weeks aerobic and Resistance training on AMP-activated protein kinase (AMPK) gene expression in soleus muscle and insulin resistance of STZ-induced diabetic rat.<br>
Methods: The research method of present study was experimental. For this purpose, 21 male wistar rat with 200 to 250 g weight and 8 to 10 weeks old were selected. Then the induction of type II diabetes was performed by intraperitoneal injection of Streptozotocin. The samples were randomly allocated to diabetes control, diabetes resistance training and diabetes aerobic training groups. Resistance training was scheduled for 10 repetitions of climbing a ladder carrying a load 100% of body weight, and aerobic training protocol was running on a treadmill with 25 m/min for 40 min and both training continuing for 8 weeks long and 5 day/week. 48 hours after last training session, soleus muscle isolated and AMPK gene expression was measured. For statistical data analysis, independent t-test at P<0.05 significant level was used.<br>
Results: Our study showed that the AMPK gene expression in soleus muscle of aerobic and resistance training group was significantly higher than control group (P value respectively 0.035 and 0.019). But there was no significant difference between levels of this factor in aerobic and Resistance training group (P=0.954). Fasting blood glucose level in aerobic and Resistance training group was significantly lower than control group (P=0.001). Also insulin and insulin resistance of aerobic and Resistance training group was significantly higher than control group (P<0.05).<br>
Conclusion: This results show that aerobic and resistance training trough increasing gene expression of AMPK can improve blood glucose level in type 2 diabetic rat. So It can be said that aerobic and resistance training activates an noninsulin-dependent pathway and improves insulin resistance in diabetic rats.
دیابت نوع 2, مقاومت به انسولین, بیان ژن AMPK, عضله نعلی, تمرین مقاومتی و هوازی
Type 2 diabetes, Insulin resistance, AMPK gene expression, Resistance training, Aerobic training,
81
87
http://jmciri.ir/browse.php?a_code=A-10-1-2607&slc_lang=fa&sid=1
دکتر سجاد
ارشدی
10031947532846005534
10031947532846005534
Yes
دکتر مجید
حسن قمی
10031947532846005535
10031947532846005535
No
دکتر عبدالعلی
بنایی فر
10031947532846005536
10031947532846005536
No
یاسر
کاظم زاده
10031947532846005537
10031947532846005537
No