study was conducted to determine the frequency of Staphylococcus aureus and Pseudomonas aeruginosa and determine the antibiotic susceptibility patterns in patients with acute middle ear infections referred to Amir Alam hospital.

Material and Methods: In total 102 middle ear discharge specimens were collected during 6 months period from patients with acute otitis media. Specimens were assessed for isolation and identification by microscopic examination and culture. The antibiotic susceptibility test was done by disk diffusion method of Kirby-Bauer and sensitivity frequency of tested antibiotics was assessed by appraisal diameter of the growth inhibitory zone.

Results: Of 102 tested specimens, 90 (88.2%) were recognized as Staphylococcus 27(26.5%), Streptococcus 7(6.9%), Moraxella catarrhalis 2(2%), Proteus 11(10.8%), Streptococcus pneumonia 15(14.7%), Pseudomonas15 (14.7%), Corynebacterium 6(5.9%), Haemophilus influenza 7(6.9%) and 12(11.8%) specimens were non cultureable. Almost all the isolated bacteria to antibiotics that are commonly prescribed to patients by Physician routinely including penicillin, ampicillin, and amoxicillin showed resistance.

Conclusion: According to the emergence of resistant strains to conventional antibiotic therapy, especially beta-lactam antibiotics and erythromycin, it is recommended to perform further studies to determine full picture of antibiotic susceptibility in circulating in patients with AOM to revise existing guidelines of empirical therapy of AOM in Iran.

Key words: Acute Otitis media, Antibiogram, Staphylococcus aureus, Pseudomonas aeruginosa