Journal of Medical Council of Iran
مجله علمی پژوهشی سازمان نظام پزشکی
Volume 37, Issue 2 (2019)
jmciri 2019, 37(2): 110-116 |
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The Effect of Intestinal Microbiota Metabolites on HT29 Cell line by Using MTT Method in Patients with Colorectal Cancer. jmciri 2019; 37 (2) :110-116
URL: http://jmciri.ir/article-1-2909-en.html
URL: http://jmciri.ir/article-1-2909-en.html
Abstract: (3294 Views)
Background: Colorectal cancer is one of the most common malignant tumors, Human guts harbor abundant microbes that adjust many aspects of host physiology. Increasing studies show that gut microbiota plays a significant role in the incidence and expansion of CRC, as a result of virulence factors, bacterial metabolites, or inflammatory pathways.
Materials and Methods: In this study, viability of HT29 cells, treated by different time of microbiota metabolits (3,6,12, 18h), was assessed by MTT assay respectively.
Results: Treated cells with microbiota metabolits showed increased viability and proliferation, in time-dependent analysis by MTT assay.
Conclusion: Considering that microbial metabolits is able to induce proliferation and increase cell viability, it seems microbial metabolites imbalance or dysbiosis in the gut due to the dietary or environmental changes or lifestyle risk factors may cause colorectal cancer.
Materials and Methods: In this study, viability of HT29 cells, treated by different time of microbiota metabolits (3,6,12, 18h), was assessed by MTT assay respectively.
Results: Treated cells with microbiota metabolits showed increased viability and proliferation, in time-dependent analysis by MTT assay.
Conclusion: Considering that microbial metabolits is able to induce proliferation and increase cell viability, it seems microbial metabolites imbalance or dysbiosis in the gut due to the dietary or environmental changes or lifestyle risk factors may cause colorectal cancer.
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