Journal of Medical Council of Iran
مجله علمی پژوهشی سازمان نظام پزشکی
Volume 37, Issue 1 (2019)
jmciri 2019, 37(1): 20-27 |
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Forouzesh F, Haghbin M, Rostami Nejad M, Asadzadeh Aghdaei H, Amin Pourhoseingholi M. Evaluation of CXCL10 and CXCL11 Genes in Patients with Celiac Disease by Specific Primer Paris . jmciri 2019; 37 (1) :20-27
URL: http://jmciri.ir/article-1-2875-en.html
URL: http://jmciri.ir/article-1-2875-en.html
Flora Forouzesh 
, Mahrokh Haghbin , Mohammad Rostami Nejad , Hamid Asadzadeh Aghdaei , Mohammad Amin Pourhoseingholi
, Mahrokh Haghbin , Mohammad Rostami Nejad , Hamid Asadzadeh Aghdaei , Mohammad Amin Pourhoseingholi
Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Abstract: (3162 Views)
Background: Celiac Disease (CD) is a T cell-mediated disorder. Recent studies suggest the role of chemokines CXCL10 and CXCL11 to promote the arrival of cells into inflamed tissues and in lymphocytic recruitment in active CD. The aim of this study was to investigate the new specific primer pairs for analysis of human CXCL10 and CXCL11 genes in blood samples of CD patients by Polymerase Chain Reaction (PCR) technique and to examine the quantitative expression of these genes in the blood of celiac patients compared with healthy controls.
Methods: Blood samples were collected from 10 CD patients diagnosed according to the Iranian Society for Pediatric Gastroenterology and Marsh criteria and 10 as a control group after extraction of RNA from patients and cDNA was synthesized. By bioinformatics analysis, the specific regions of the CXCL10 and CXCL11 genes were designed and exon-exon junction specific primers and PCR was performed. Later gene expression analysis was performed with Real-time PCR.
Results: According to the results, all designed primers are highly capable of replicating the desired components. Also, there was a significant increase in expression of two chemokines CXCL10 and CXCL11 genes in the celiac patients compared to control groups (P<0.05).
Conclusion: Our results from this study showed that changes in the expression of CXCL10 and CXCL11 genes in celiac patients indicate the role of the inherent immune system in the pathogenesis of CD.
Methods: Blood samples were collected from 10 CD patients diagnosed according to the Iranian Society for Pediatric Gastroenterology and Marsh criteria and 10 as a control group after extraction of RNA from patients and cDNA was synthesized. By bioinformatics analysis, the specific regions of the CXCL10 and CXCL11 genes were designed and exon-exon junction specific primers and PCR was performed. Later gene expression analysis was performed with Real-time PCR.
Results: According to the results, all designed primers are highly capable of replicating the desired components. Also, there was a significant increase in expression of two chemokines CXCL10 and CXCL11 genes in the celiac patients compared to control groups (P<0.05).
Conclusion: Our results from this study showed that changes in the expression of CXCL10 and CXCL11 genes in celiac patients indicate the role of the inherent immune system in the pathogenesis of CD.
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